Biodegradation of Malachite Green by Extracellular Laccase Producing Bacillus thuringiensis RUN1

A bacteria strain Bacillus thuringiensis RUN1identifiedby 16s RNA gene phylogenetic analysiswas used to decolorize malachite green, a triphenylmethane dye in simulated wastewater. The ability of the organism produce extracellular laccase and degrade were also investigated. Results showed that decolorized (84.67 ± 1.19 %) green at 40 mgl-1 within 6 h; decolorization was associated production by experimentally. Laccase activity increased as process progressed, with highest value 0.1043 0.02 U/min/ mg protein recorded after 24 h incubation using ABTS substrate. In addition, FTIR analysis actually degraded dye. It therefore concluded this will be relevant biotreatment industrial effluent containing laccase, an industrially important enzyme.